Recommended reading from our members, March 2018

Collected here are this months publications from SLTB members and related groups. Please contact SLTB if you'd like your research to appear as a featured publication or in next month's listings. Also, let us know if this is interesting or useful for you so we can keep producing these for our members! 

These new publications were released in the last 2-3 months.




Topic: Vitrification of mammalian tissue, Biobanking, reproductive technologies


Ovarian tissue cryopreservation by stepped vitrification and monitored by X-ray computed tomography.



Ovarian tissue cryopreservation is, in most cases, the only fertility preservation option available for female patients soon to undergo gonadotoxic treatment. To date, cryopreservation of ovarian tissue has been carried out by both traditional slow freezing method and vitrification, but even with the best techniques, there is still a considerable loss of follicle viability. In this report, we investigated a stepped cryopreservation procedure which combines features of slow cooling and vitrification (hereafter called stepped vitrification). Bovine ovarian tissue was used as a tissue model. Stepwise increments of the Me2SO concentration coupled with stepwise drops-in temperature in a device specifically designed for this purpose and X-ray computed tomography were combined to investigate loading times at each step, by monitoring the attenuation of the radiation proportional to Me2SO permeation. Viability analysis was performed in warmed tissues by immunohistochemistry. Although further viability tests should be conducted after transplantation, preliminary results are very promising. Four protocols were explored. Two of them showed a poor permeation of the vitrification solution (P1 and P2). The other two (P3 and P4), with higher permeation, were studied in deeper detail. Out of these two protocols, P4, with a longer permeation time at -40 °C, showed the same histological integrity after warming as fresh controls.

Cryobiology. 2018 Mar 5. pii: S0011-2240(17)30357-7. doi: 10.1016/j.cryobiol.2018.03.001. [Epub ahead of print]

Corral A(1), Clavero M(2), Gallardo M(3), Balcerzyk M(1), Amorim CA(4), Parrado-Gallego Á(1), Dolmans MM(5), Paulini F(4), Morris J(6), Risco R(7).

Author information:  1 Centro Nacional de Aceleradores (Universidad de Sevilla-CSIC-Junta de Andalucía), Calle Thomas Alva Edison 7, 41092, Sevilla, Spain. 2 Departamento de Física Aplicada III, Escuela Técnica Superior de Ingeniería, Universidad de Sevilla, Camino Descubrimientos s/n, Isla Cartuja, 41092, Sevilla, Spain. 3 Ginemed Clínicas Sevilla, Calle Farmaceutico Murillo Herrera 3, 41010, Sevilla, Spain. 4 Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Mounier 52, Bte. B1.52.02, 1200, Brussels, Belgium.  5 Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Mounier 52, Bte. B1.52.02, 1200, Brussels, Belgium; Cliniques Universitaires Saint-Luc, Gynecology Department, Avenue Hippocrate 10, 1200, Brussels, Belgium. 6 Asymptote Limited, GE Healthcare, Sovereign House, Chivers Way, Vision Park, Cambridge, CB24 9ZR, United Kingdom. 7 Centro Nacional de Aceleradores (Universidad de Sevilla-CSIC-Junta de Andalucía), Calle Thomas Alva Edison 7, 41092, Sevilla, Spain; Departamento de Física Aplicada III, Escuela Técnica Superior de Ingeniería, Universidad de Sevilla, Camino Descubrimientos s/n, Isla Cartuja, 41092, Sevilla, Spain.

Electronic address:


Low Temperature-related publications from SLTB members:



Habitat-related seed germination traits in alpine habitats.

Ecol Evol. 2017 Nov 24;8(1):150-161. doi: 10.1002/ece3.3539. eCollection 2018 Jan.

Tudela-Isanta M(1), Fernández-Pascual E(2,3), Wijayasinghe M(1), Orsenigo S(4), Rossi G(1), Pritchard HW(3), Mondoni A(1).

Author information: 1 Department of Earth and Environmental Sciences University of Pavia Pavia Italy. 2 Department of Biological Sciences George Washington University Washington DC USA. 3 Comparative Plant and Fungal Biology Royal Botanic Gardens Ardingly UK. 4 Department of Agricultural and Environmental Sciences University of Milan Milano Italy.


Understanding the key aspects of plant regeneration from seeds is crucial in assessing species assembly to their habitats. However, the regenerative traits of seed dormancy and germination are underrepresented in this context. In the alpine zone, the large species and microhabitat diversity provide an ideal context to assess habitat-related regenerative strategies. To this end, seeds of 53 species growing in alpine siliceous and calcareous habitats (6230 and 6170 of EU Directive 92/43, respectively) were exposed to different temperature treatments under controlled laboratory conditions. Germination strategies in each habitat were identified by clustering with k-means. Then, phylogenetic least squares correlations (PGLS) were fitted to assess germination and dormancy differences between species' main habitat (calcareous and siliceous), microhabitat (grasslands, heaths, rocky, and species with no specific microhabitats), and chorology (arctic-alpine and continental). Calcareous and siliceous grasslands significantly differ in their germination behaviour with a slow, mostly overwinter germination and high germination under all conditions, respectively. Species with high overwinter germination occurs mostly in heaths and have an arctic-alpine distribution. Meanwhile, species with low or high germinability in general inhabit in grasslands or have no specific microhabitat (they belong to generalist), respectively. Alpine species use different germination strategies depending on habitat provenance, species' main microhabitat, and chorotype. Such differences may reflect adaptations to local environmental conditions and highlight the functional role of germination and dormancy in community ecology.





Transplanted human thymus slices induce and support T-cell development in mice after cryopreservation.

Eur J Immunol. 2018 Jan 10. doi: 10.1002/eji.201747193. [Epub ahead of print]

Ross S(1), Cheung M(1), Lau CI(1), Sebire N(2), Burch M(2), Kilbride P(3), Fuller B(4), Morris GJ(3), Davies EG(1,2), Crompton T(1).

Author information: 1 UCL Great Ormond Street Institute of Child Health, London, UK. 2 Great Ormond Street Hospital, London, UK. 3 Asymptote Ltd, Sovereign House, Vision Park, Cambridge, UK. 4 UCL Division of Surgery and Interventional Science, Royal Free Hospital Campus, London, UK.


Here we show that slices of human thymus tissue that have been frozen and thawed can induce and support T-cell development when transplanted into nude mice. Babies born without a thymus require urgent treatment to reconstitute T-cell immunity. Thymus tissue is removed from infants during cardiac surgery, to allow access to the heart. This discarded thymus tissue can be transplanted into athymic infants to reconstitute T-cell immunity. Slices of thymus tissue are transplanted into the thigh, after a two-three week culture period to deplete thymocytes. This procedure is life-saving, but recipients have low T-cell counts, and may develop autoimmunity. It is not possible to attempt to MHC-match transplants between-donor and recipient because of the urgency of performing the procedure. As delays in thymus transplantation could be life-threatening, the procedure would be improved if it were possible to freeze thymus slices for transplantation. Cryopreservation would also open up the possibility of partial MHC-matching. We adapted a slow cooling protocol based on cryopreservation of ovarian tissue fragments to freeze slices of human thymus of 1mm thickness and showed that on thawing such slices can induce and support T-cell development in vivo in an animal model. This article is protected by copyright. All rights reserved.




Mitochondrial Impairment as a Key Factor for the Lack of Attachment after Cold Storage of Hepatocyte Suspensions.

Cell Transplant. 2017 Dec;26(12):1855-1867. doi: 10.1177/0963689717743254.

Pless-Petig G(1), Walter B(1), Bienholz A(2), Rauen U(1).

Author information: 1Institut für Physiologische Chemie, Universitätsklinikum Essen, Essen, Germany. 2 Klinik für Nephrologie, Universitätsklinikum Essen, Essen, Germany.


Isolated primary hepatocytes, which are widely used for pharmacological and clinical purposes, usually undergo certain periods of cold storage in suspension during processing. While adherent hepatocytes were shown previously to suffer iron-dependent cell death during cold (4 °C) storage and early rewarming, we previously found little iron-dependent hepatocyte death in suspension but severely decreased attachment ability unless iron chelators were added. Here, we focus on the role of mitochondrial impairment in this nonattachment of hepatocyte suspensions. Rat hepatocyte suspensions were stored in a chloride-poor, glycine-containing cold storage solution with and without iron chelators at 4 °C. After 1 wk of cold storage in the basic cold storage solution, cell viability in suspension was unchanged, while cell attachment was decreased by >80%. In the stored cells, a loss of mitochondrial membrane potential (MMP), a decrease in adenosine triphosphate (ATP) content (2 ± 2 nmol/106 cells after cold storage, 5 ± 3 nmol/106 cells after rewarming vs. control 29 ± 6 nmol/106 cells), and a decrease in oxygen consumption (101 ± 59 pmol sec-1 per 106 cells after rewarming vs. control 232 ± 83 pmol sec-1 per 106 cells) were observed. Addition of iron chelators to the cold storage solution increased cell attachment to 53% ± 20% and protected against loss of MMP, and cells were able to partially regenerate ATP during rewarming (15 ± 10 nmol/106 cells). Increased attachment could also be achieved by addition of the inhibitor combination of mitochondrial permeability transition, trifluoperazine + fructose. Attached hepatocytes displayed normal MMP and mitochondrial morphology. Additional experiments with freshly isolated hepatocytes confirmed that impaired energy production-as elicited by an inhibitor of the respiratory chain, antimycin A-can decrease cell attachment without decreasing viability. Taken together, these results suggest that mitochondrial impairment with subsequent energy deficiency is a key factor for the lack of attachment of cold-stored hepatocyte suspensions.




Plant temperature acclimation and growth rely on cytosolic ribosome biogenesis factor homologs.

Plant Physiol. 2018 Jan 30. pii: pp.01448.2017. doi: 10.1104/pp.17.01448. [Epub ahead of print]

Beine-Golovchuk O(1), Pereira Firmino AA(1), Dąbrowska A(1), Schmidt S(1), Erban A(2), Walther D(3), Zuther E(4), Hincha DK(5), Kopka J(6).

Author information: 1 Max Planck Institute of Molecular Plant Physiology CITY: Potsdam-Golm STATE: Br Germany [DE]. 2 Max Planck Institute for Molecular Plant Physiology CITY: Potsdam.Golm Germany [DE]. 3 MPIMP N/A CITY: N/A POSTAL_CODE: N/A Germany [DE]. 4 Max-Planck-Institute for Plant Physiology CITY: Potsdam POSTAL_CODE: 14476 Germany [DE]. 5 Max-Planck-Institut für Molekulare Pflanzenphysiologie CITY: Potsdam POSTAL_CODE: D-14476 Germany [DE]. 6 Max Planck Institute of Molecular Plant Physiology CITY: Potsdam-Golm STATE: Br POSTAL_CODE: 14476 Germany [DE]


Arabidopsis REI1-LIKE (REIL) proteins, REIL1 and REIL2, are homologs of a yeast ribosome biogenesis factor (RBF) that participates in late cytoplasmic steps of 60S ribosomal subunit maturation. Strong growth inhibition at 10{degree sign}C of the reil1-1 reil2-1 mutant was complemented by expression of N-terminal FLUORESCENT PROTEIN (FP)-REIL fusion proteins under control of the UBIQUITIN 10 promoter. Arabidopsis REIL1 has a conserved function as was demonstrated by cytosolic localization of FP-REIL1 and native REIL1 interaction with the 60S subunit of Col-0 wildtype. In contrast to the yeast homologs, REIL1 was also present in translating ribosome fractions. Systems analysis revealed that Col-0 remodels the cytosolic translation machinery after shift to 10{degree sign}C by accumulation of cytosolic ribosome subunits and by induced expression of cytosolic rRNA, ribosomal genes, RBFs, and translation initiation- or elongation-factors. In reil1-1 reil2-1 all processes were delayed in association with inhibited growth, but maintained cellular integrity or acquired freezing tolerance. Further analysis linked REIL function to plant specific processes. For example, non-acclimated reil1-1 reil2-1 triggered cold-acclimation responses that included activation of the DREB/CBF-regulon, namely CBF1/DREB1B, CBF2/DREB1C, and CBF3/DREB1A. REIL function may therefore feedback on temperature perception by suppression of premature cold-acclimation at standard temperature. Also, acclimated reil1-1 reil2-1 did not activate FLOWERING LOCUS T expression in mature leaves. In conclusion, we suggest a role of REIL proteins for cold induced plant ribosome remodeling. Arabidopsis REIL proteins appear to enhance accumulation of cytosolic ribosome subunits after cold-shift either by de novo synthesis or by recycling from the translating ribosome fraction.




Isobaric Vapor-Liquid Phase Diagrams for Multicomponent Systems with Nanoscale Radii of Curvature.

J Phys Chem B. 2018 Mar 1;122(8):2434-2447. doi: 10.1021/acs.jpcb.8b00167. Epub 2018 Feb 14.

Shardt N(1), Elliott JAW(1).

Author information: 1 Department of Chemical and Materials Engineering, University of Alberta , Edmonton, Alberta T6G 1H9, Canada.


At any given temperature, pressure, and composition, a compound or a mixture of compounds will exist either in a single phase, whether solid, liquid, or vapor, or in a combination of these phases coexisting in equilibrium. For multiphase systems, it is known that the geometry of the interface impacts the equilibrium state; this effect has been well-studied in single component systems with spherical interfaces. However, multicomponent phase diagrams are usually calculated assuming a planar interface between phases. Recent experimental and theoretical work has started to investigate the effect of curved interfaces on multicomponent phase equilibrium, but these analyses have been limited to isothermal conditions or to a portion of the isobaric phase diagram. Herein, we consider complete vapor-liquid phase diagrams (both bubble and dew lines) under isobaric conditions. We use Gibbsian composite-system thermodynamics to derive the equations governing vapor-liquid equilibrium for systems with a spherical interface separating the phases. We validate our approach by comparing the predicted nitrogen/argon dew points with reported literature data. We then predict complete isobaric phase diagrams as a function of radius of curvature for an ideal methanol/ethanol system and for a nonideal ethanol/water system. We also determine how the azeotropic composition of ethanol/water changes. The effect of curvature on isobaric phase diagrams is similar to that seen on isothermal phase diagrams. This work extends the study of curved-interface multicomponent phase equilibrium to isobaric systems, expanding the conditions under which nanoscale systems, such as nanofluidic systems, shale gas reservoirs, and cloud condensation nuclei, can be understood.




Biospecimens and Biobanking in Global Health.

Clin Lab Med. 2018 Mar;38(1):183-207. doi: 10.1016/j.cll.2017.10.015. Epub 2017 Dec 24.

Mendy M(1), Lawlor RT(2), van Kappel AL(3), Riegman PHJ(4), Betsou F(5), Cohen OD(6), Henderson MK(7).

Author information: 1 Laboratory Services and Biobank Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, Lyon 69372, France. 2 ARC-Net Applied Research on Cancer Centre, University of Verona, Piazzale LA Scuro 10, Verona 37134, Italy. 3 IMV Technologies, ZI n°1 Est, l'Aigle 61300, France. 4 Department of Pathology, Tissue Bank, Erasmus MC, Dr Molewaterplein 40, Rotterdam 3015, The Netherlands. 5 Integrated BioBank of Luxembourg, 6 rue Nicolas Ernest Barble, Luxembourg L-1210, Luxembourg. 6 AGEIS EA 7407 Laboratory, Medical School of Grenoble, Joseph Fourier University, Domaine de la Merci, La Tronche 38700, France. 7 Center for Global Health, National Cancer Institute, NIH, DHHS, 9609 Medical Center Drive, Room 3W534, Bethesda, MD 20892, USA. Electronic address:


Biobanks provide a critical infrastructure to support research in human health. Biospecimens and their accompanying data are increasingly needed to support biomedical research and clinical care. The original text was initially published in the Handbook for Cancer Research in Africa. The value of this publication is great as it underlines the importance of biobanks in Africa as a key resource to increase quality scientific research and participate in global health research. Therefore, a revision to extend these principles to other low resource contexts, to include updated material and references and add the topic of biobank sustainability were relevant.




Directional freezing for the cryopreservation of adherent mammalian cells on a substrate.

PLoS One. 2018 Feb 15;13(2):e0192265. doi: 10.1371/journal.pone.0192265. eCollection 2018.

Bahari L(1), Bein A(1), Yashunsky V(1), Braslavsky I(1).

Author information: 1 The Hebrew University of Jerusalem, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel.


Successfully cryopreserving cells adhered to a substrate would facilitate the growth of a vital confluent cell culture after thawing while dramatically shortening the post-thaw culturing time. Herein we propose a controlled slow cooling method combining initial directional freezing followed by gradual cooling down to -80°C for robust preservation of cell monolayers adherent to a substrate. Using computer controlled cryostages we examined the effect of cooling rates and dimethylsulfoxide (DMSO) concentration on cell survival and established an optimal cryopreservation protocol. Experimental results show the highest post-thawing viability for directional ice growth at a speed of 30 μm/sec (equivalent to freezing rate of 3.8°C/min), followed by gradual cooling of the sample with decreasing rate of 0.5°C/min. Efficient cryopreservation of three widely used epithelial cell lines: IEC-18, HeLa, and Caco-2, provides proof-of-concept support for this new freezing protocol applied to adherent cells. This method is highly reproducible, significantly increases the post-thaw cell viability and can be readily applied for cryopreservation of cellular cultures in microfluidic devices.




Vitrification of one-cell mouse embryos in cryotubes.

Cryobiology. 2018 Feb 8. pii: S0011-2240(17)30561-8. doi: 10.1016/j.cryobiol.2018.01.013. [Epub ahead of print]

Seki S1, Basaki K2, Komatsu Y2, Fukuda Y2, Yano M2, Matsuo Y2, Obata T2, Matsuda Y2, Nishijima K2.

Author information: 1 Experimental Animal Division, Bioscience Education and Research Support Center, Akita University, 1-1-1 Hondo, Akita, Akita, 010-8543, Japan. Electronic address: 2 Experimental Animal Division, Bioscience Education and Research Support Center, Akita University, 1-1-1 Hondo, Akita, Akita, 010-8543, Japan.


Preventing intracellular ice formation is essential to cryopreserve cells. Prevention can be achieved by converting cell water into a non-crystalline glass, that is, to vitrify. The prevailing belief is that to achieve vitrification, cells must be suspended in a solution containing a high concentration of glass-inducing solutes and cooled rapidly. In this study, we vitrified 1-cell mouse embryos and examined the effect of the cooling rate, the warming rate, and the concentration of cryoprotectant on cell survival. Embryos were vitrified in cryotubes. The vitrification solutions used were EFS20, EFS30, and EFS40, which contained ethylene glycol (20, 30 and 40% v/v, respectively), Ficoll (24%, 21%, and 18% w/v, respectively) and sucrose (0.4 0.35, and 0.3 M, respectively). A 5-μl EFS solution suspended with 1-cell embryos was placed in a cryotube. After 2 min in an EFS solution at 23 °C, embryos were vitrified by direct immersion into liquid nitrogen. The sample was warmed at 34 °C/min, 4,600 °C/min and 6,600 °C/min. With EFS40, the survival was low regardless of the warming rate. With EFS30 and EFS20, survival was also low when the warming rate was low, but increased with higher warming rates, likely due to prevention of intracellular ice formation. When 1-cell embryos were vitrified with EFS20 and warmed rapidly, almost all of the embryos developed to blastocysts in vitro. Moreover, when vitrified 1-cell embryos were transferred to recipients at the 2-cell stage, 43% of them developed to term. In conclusion, we developed a vitrification method for 1-cell mouse embryos by rapid warming using cryotubes.




Organization model for allotransplantations of cryopreserved vascular grafts in Czech Republic.

Cell Tissue Bank. 2018 Mar 3. doi: 10.1007/s10561-018-9691-4. [Epub ahead of print]

Špaček M1, Měřička P2, Janoušek L3, Štádler P4, Adamec M5, Vlachovský R6, Guňka I7,8, Navrátil P8, Thieme F3, Mitáš P9 , Špunda R9, Špatenka J10, Staffa R6, Němec P11, Lindner J9.

Author information: 1 2nd Department of Surgery - Department of Cardiovascular Surgery, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic. 2 Tissue Bank, University Hospital Hradec Králové, Hradec Králové, Czech Republic. 3 Department of Transplant Surgery, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. 4 Department of Vascular Surgery, Na Homolce Hospital, Prague, Czech Republic. 5 Transplants Coordinating Centre, Prague, Czech Republic. 6 2nd Department of Surgery, St. Anne's University Hospital, Faculty of Medicine, Masaryk Univirsity, Brno, Czech Republic. 7 Department of Surgery, University Hospital and Faculty of Medicine Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic. 8 Transplant Center, University Hospital and Faculty of Medicine Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic. 9 2nd Department of Surgery - Department of Cardiovascular Surgery, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic. 10 Department of Transplantation and Tissue Bank, University Hospital Motol, Prague, Czech Republic. 11 Center of Cardiovascular Surgery and Transplantations Brno, Brno, Czech Republic.


The transplantation of fresh or cryopreserved vascular allografts in patients with a prosthetic graft infection or critical limb ischemia is necessary for their limb salvage and, in many cases, represents a lifesaving procedure. While transplantation of fresh allografts has a long history in the Czech Republic, the standard use of cryopreserved vascular allografts was introduced into the clinical practice in 2011 as a result of the implementation of EU Directive 2004/23/EC into national legislation (Human Cell and Tissue Act No. 296/2008 Coll.). The authors present an organizational model based on cooperation between the majority of Czech Transplant Centers with a tissue establishment licensed by the national competent authority. In various points, we are addressing individual aspects of experimental and clinical studies which affect clinical practice. Based on experimental and clinical work, the first validation of cryopreserved arterial and venous grafts for clinical use was performed between 2011 and 2013. The growing number of centers participating in this programme led to a growing number of patients who underwent transplantation of vascular allografts. In 2015 the numbers of transplanted fresh versus cryopreserved allografts in the Czech Republic were almost equal. Cooperation of the participating centers in the Czech Republic with the licensed Tissue Establishment made it possible to achieve a full compliance with the European Union Directives, and harmonized national legal norms and assured a high quality of cryopreserved vascular allografts.




Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells.

Stem Cell Res Ther. 2017 Mar 11;8(1):66. doi: 10.1186/s13287-017-0512-7.

Pogozhykh D1,2, Pogozhykh O3,4, Prokopyuk V4, Kuleshova L4, Goltsev A4, Blasczyk R3, Mueller T3,5.

Author information: 1 Institute for Transfusion Medicine, Hannover Medical School, Carl-Neuberg-Straße 1, 30625, Hannover, Germany. 2 Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Pereyaslavskaya Str. 23, 61015, Kharkiv, Ukraine. 3 Institute for Transfusion Medicine, Hannover Medical School, Carl-Neuberg-Straße 1, 30625, Hannover, Germany. 4 Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Pereyaslavskaya Str. 23, 61015, Kharkiv, Ukraine. 5 Synlab Medical Care Center Weiden Ltd., Zur Kesselschmiede 4, 92637, Weiden in der Oberpfalz, Germany.




Successful implementation of rapidly advancing regenerative medicine approaches has led to high demand for readily available cellular suspensions. In particular, multipotent stromal cells (MSCs) of placental origin have shown therapeutic efficiency in the treatment of numerous pathologies of varied etiology. Up to now, cryopreservation is the only effective way to preserve the viability and unique properties of such cells in the long term. However, practical biobanking is often associated with repeated temperature fluctuations or interruption of a cold chain due to various technical, transportation, and stocking events. While biochemical processes are expected to be suspended during cryopreservation, such temperature fluctuations may lead to accumulation of stress as well as to periodic release of water fractions in the samples, possibly leading to damage during long-term storage.


In this study, we performed a comprehensive analysis of changes in cell survival, vital parameters, and differentiation potential, as well as transgene expression of placental MSCs after temperature fluctuations within the liquid nitrogen steam storage, mimicking long-term preservation in practical biobanking, transportation, and temporal storage.


It was shown that viability and metabolic parameters of placental MSCs did not significantly differ after temperature fluctuations in the range from -196 °C to -100 °C in less than 20 cycles in comparison to constant temperature storage. However, increasing the temperature range to -80 °C as well as increasing the number of cycles leads to significant lowering of these parameters after thawing. The number of apoptotic changes increases depending on the number of cycles of temperature fluctuations. Besides, adhesive properties of the cells after thawing are significantly compromised in the samples subjected to temperature fluctuations during storage. Differentiation potential of placental MSCs was not compromised after cryopreservation with constant end temperatures or with temperature fluctuations. However, regulation of various genes after cryopreservation procedures significantly varies. Interestingly, transgene expression was not compromised in any of the studied samples.


Alterations in structural and functional parameters of placental MSCs after long-term preservation should be considered in practical biobanking due to potential temperature fluctuations in samples. At the same time, differentiation potential and transgene expression are not compromised during studied storage conditions, while variation in gene regulation is observed.





Less Low Temperature-related research from SLTB members:


Chemical characterization of 21 species of marine macroalgae common in Norwegian waters: benefits of and limitations to their potential use in food and feed.

J Sci Food Agric. 2017 Nov 28. doi: 10.1002/jsfa.8798. [Epub ahead of print]

Irene B1,2, Ikram B1, Christian BG3, Nina LS1, Rune W1,2, Heidi A1, Svenja H4, Erik-Jan L1.


Media Screening for Obtaining Haematococcus pluvialis Red Motile Macrozooids Rich in Astaxanthin and Fatty Acids.

Biology (Basel). 2017 Dec 26;7(1). pii: E2. doi: 10.3390/biology7010002.

Butler TO1,2, McDougall GJ3, Campbell R4, Stanley MS5, Day JG6.


Preclinical imaging methods for assessing the safety and efficacy of regenerative medicine therapies.

NPJ Regen Med. 2017 Oct 19;2:28. doi: 10.1038/s41536-017-0029-9. eCollection 2017.

Scarfe L, Brillant N, Kumar JD, Ali N, Alrumayh A, Amali M, Barbellion S, Jones V, Niemeijer M, Potdevin S, Roussignol G, Vaganov A, Barbaric I, Barrow M, Burton NC, Connell J, Dazzi F, Edsbagge J, French NS, Holder J, Hutchinson C, Jones DR, Kalber T, Lovatt C, Lythgoe MF, Patel S, Patrick PS, Piner J, Reinhardt J, Ricci E, Sidaway J, Stacey GN, Starkey Lewis PJ, Sullivan G, Taylor A, Wilm B, Poptani H, Murray P, Goldring CEP, Park BK.


Common genetic variants associated with pancreatic adenocarcinoma may also modify risk of pancreatic neuroendocrine neoplasms.

Carcinogenesis. 2017 Dec 22. doi: 10.1093/carcin/bgx150. [Epub ahead of print]

Obazee O, Capurso G, Tavano F, Archibugi L, De Bonis A, Greenhalf W, Key T, Pasquali C, Milanetto AC, Hackert T, Fogar P, Liço V, Dervenis C, Lawlor RT, Landoni L, Gazouli M, Zambon CF, Funel N, Strobel O, Jamroziak K, Cantù C, Malecka-Panas E, Landi S, Neoptolemos JP, Basso D, Talar-Wojnarowska R, Rinzivillo M, Andriulli A, Canzian F, Campa D.

Attachment of nanoparticulate drug-release systems on poly(ε-caprolactone) nanofibers via a graftpolymer as interlayer.

Colloids Surf B Biointerfaces. 2017 Dec 29;163:309-320. doi: 10.1016/j.colsurfb.2017.12.050. [Epub ahead of print]

de Cassan D, Sydow S, Schmidt N, Behrens P, Roger Y, Hoffmann A, Hoheisel AL, Glasmacher B, Hänsch R, Menzel H.


Preparation and Characterization of Antimicrobial Films Based on LDPE/Ag Nanoparticles with Potential Uses in Food and Health Industries.

Nanomaterials (Basel). 2018 Jan 24;8(2). pii: E60. doi: 10.3390/nano8020060.

Olmos D, Pontes-Quero GM, Corral A, González-Gaitano G, González-Benito J.


Utilizing Fibrin-Alginate and Matrigel-Alginate for Mouse Follicle Development in Three-Dimensional Culture Systems.

Biopreserv Biobank. 2018 Jan 24. doi: 10.1089/bio.2017.0087. [Epub ahead of print]

Sadr SZ, Fatehi R, Maroufizadeh S, Amorim CA, Ebrahimi B.


Cellular senescence: immunosurveillance and future immunotherapy.

Ageing Res Rev. 2018 Feb 7. pii: S1568-1637(18)30011-4. doi: 10.1016/j.arr.2018.02.001. [Epub ahead of print]

Burton DGA, Stolzing A.


A draft map of the human ovarian proteome for tissue engineering and clinical applications.

Mol Cell Proteomics. 2018 Feb 23. pii: mcp.RA117.000469. doi:10.1074/mcp.RA117.000469. [Epub ahead of print]

Ouni E, Vertommen D, Chiti MC, Dolmans MM, Amorim CA.


A selective c-Fos/AP-1 inhibitor prevents cartilage destruction and subsequent osteophyte formation.

Biochem Biophys Res Commun. 2018 Feb 21. pii: S0006-291X(18)30384-X. doi: 10.1016/j.bbrc.2018.02.147. [Epub ahead of print]

Motomura H, Seki S, Shiozawa S, Aikawa Y, Nogami M, Kimura T.


Anaemia in patients with chronic liver disease and its association with morbidity and mortality following liver transplantation.

Int J Surg. 2018 Feb 27. pii: S1743-9191(18)30584-3. doi: 10.1016/j.ijsu.2018.02.053. [Epub ahead of print]

Collas O, Robertson FP, Fuller BJ, Davidson BR.


Dose Escalation of Vitamin D3 Yields Similar Cryosurgical Outcome to Single Dose Exposure in a Prostate Cancer Model.

Cancer Control. 2018 Jan-Mar;25(1):1073274818757418. doi: 10.1177/1073274818757418.

Santucci KL, Baust JM, Snyder KK, Van Buskirk RG, Baust JG.


Making the most of sperm activation responses: experiments with boar spermatozoa and bicarbonate.

Reprod Fertil Dev. 2018 Mar 8. doi: 10.1071/RD17476. [Epub ahead of print]

Holt WV, Satake N.